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iv Kit Contents and Storage Shipping and Storage The TOPO TA Cloning¤ Kit is shipped on dry ice Each kit contains a box with TOPO TA Cloning¤ reagents Box 1 and a box with TOP10 or TOP10F One Shot competent cells Box 2 Store Box 1 at 20¡C and Box 2 at 80¡C Types of TOPO
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TOPO TA Cloning ¤ Version E 180714 25 Technical Service ¥ Spread 40˚µl of 40 mg/ml X gal on each LB plate and incubate at 37¡C until ready for use
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Students should wear goggles and aprons or lab coats during the activity and exercise due caution around Bunsen burners or hot plates Inform students that cooking oil boils at a higher temperature than water 225° C and have them cover their beakers with aluminum foil to contain the popping corn and boiling oil
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The volcanic country of Iceland which straddles the Mid Atlantic Ridge offers scientists a natural laboratory for studying on land the processes also occurring along the submerged parts of a spreading ridge Iceland is splitting along the spreading center between the North American and Eurasian Plates as North America moves westward
Chapter 4 Structural Modeling and Analysis 4 i CHAPTER 4 STRUCTURAL MODELING AND ANALYSIS Plate Element A plate element is a two dimensional solid element that acts like a flat plate There are two out of plane rotations and the normal displacement as Degree of Freedom DOF These elements model plate
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This test method extends to the atmospheric distillation of petroleum products using a laboratory batch distillation unit plates or trays used in distillation
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Cut 2mm of tail and place into an Eppendorf tube or 96 well plate Add 75ul 25mM NaOH / mM EDTA Place in thermocycler at 98ºC for 1 hour then reduce the temperature to 15°C until ready to proceed to the next step
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